Phenotypic Changes in Diabetic Neuropathy Induced by a High-Fat Diet in Diabetic C57Bl/6 Mice
Figure 5
Intraepidermal Nerve Fiber Density. Plantar skin of the hindpaw was dissected at 8 weeks following STZ. Immunofluorescence staining for PGP 9.5 was used to visualize and count nerve fibers that cross the epidermal/dermal border. (a-b): Representative images showing a section of plantar skin used to quantify IENFD. (a) Nondiabetic Standard Diet. (b) Diabetic Standard Diet. Arrows indicate individual axons within the epidermis. Scale bar = 50 μM. (c) Quantification of IENFD ( = 11-12 mice per group). Data presented as means ± SEM. *.
