Involvement of NF-κB/IL-6 Pathway in the Processing of Colorectal Carcinogenesis in Colitis Mice
Figure 4
The expression patterns of IL-6 and NF-κB positive cells in the tumor tissues over examined time-points. Represented immunohistochemical photos taken from CAC mice treated with DMH and DSS for 18 weeks showed that IL-6 (Figure 4(a)) and NF-κB (Figure 4(b)) immunoreactivities were observed in both the transformed epithelium (arrow head pointed in Figures 4(a) and 4(b)) and tumor stroma (arrow pointed in Figures 4(a) and 4(b)). The cell density grading results confirmed the immunohistochemical observations and demonstrated that the densities of IL-6 (Figures 4(c) and 4(d)) and NF-κB (Figures 4(e) and 4(f)) positive cells in both the CAC epithelium and tumor stroma were significantly increased over examined time-points (white bar for age matched control, black bar for dysplastic lesion at different examined time-points in Figures 4(c)–4(f)). The western blotting results revealed that both of the IL-6 and the NF-κB proteins (Figure 4(g)) were increased in dysplastic tumor tissues after induction for 14 weeks and persisted to 22 weeks and confirmed the immunohistochemical observations as well (A&B: IHC images counterstained with hematoxylin, original magnification 200x; HPF: high power field; values in Figures 4(c)–4(f) were obtained with the Mann-Whitney U test).
