Optimization of Keratin Hydrolysate Extraction from Tannery Sheep Hair Waste

Table 6

Experimental responses of the predicted values.

Run no	Temperature (°C)	Concentration (N)	Time (hr.)	Absorbance at 540 nm	Protein yield (%)
1	80	0.75	2.5	0.791	82.7
2	65	0.50	1.5	0.746	80.1
3	80	1.00	3.5	0.744	78.9
4	95	1.00	2.5	0.682	74.3
5	65	0.50	3.5	0.803	82.9
6	65	1.00	2.5	0.696	74.4
7	95	0.75	2.5	0.731	77.5
8	80	0.50	3.5	0.821	86.6
9	95	0.50	2.5	0.761	79.2
10	80	0.75	1.5	0.779	82.1
11	80	0.75	2.5	0.787	83.6
12	80	0.50	1.5	0.786	83.3
13	65	0.75	2.5	0.748	78.1
14	95	0.75	3.5	0.719	78.2
15	95	1.00	1.5	0.676	74.0
16	95	0.75	1.5	0.714	77.6
17	65	0.75	1.5	0.761	78.1
18	95	0.50	2.5	0.758	81.8
19	80	1.00	1.5	0.742	78.8
20	65	1.00	3.5	0.712	73.6