PAI-1 Expression Is Required for HDACi-Induced Proliferative Arrest in ras-Transformed Renal Epithelial Cells
Figure 6
PAI-1 antisense and sense expression vectors were used to generate the PAI-1 knockdown (4HH; PAI-1KD) and overexpressing (Rc/CMVPAI) cell lines, respectively, (a). PAI-1-deficient 4HH cells were resistant to NaB-mediated cytostasis but remained sensitive to PAI-1-induced growth arrest. The combination of NaB+PAI-1 reduced final population densities similar to that of PAI-1 alone cultures (b). Vector-driven PAI-1 overexpression in v-ras transformants also inhibited cell growth consistent with results of the PAI-1 add-back experiments (e.g., Figure 5). Data plotted represents the mean ± standard deviation for triplicate assessments of final cell densities (i.e., % confluency).
