PAI-1 Expression Is Required for HDACi-Induced Proliferative Arrest in ras-Transformed Renal Epithelial Cells
Figure 5
Increasing concentrations of PAI-1 (range 0.02 to 100 nM) effectively suppressed growth of v-ras-transformants in serum-free (a) as well as serum-supplemented (b) media. In (b), PAI-1 was added to a final level of 20 nM. PAI-1 also inhibited EGF-induced HaCaT proliferation, as assessed by crystal violet staining of cells stimulated with EGF (10 ng/mL) for 5 days in the absence or presence of PAI-1 (20 nM) (c). Growth arrest in EGF-treated HaCaT keratinocytes reflected a PAI-1-induced G1 block evident as early as 24 hours after the addition of PAI-1 compared to the prominent S-phase cohort in FBS- or control EGF-treated cultures (d). Starting quiescent populations (1 day in serum-free medium) were virtually devoid of DNA-synthesizing cells.
