Research Article

The Influence of Outer Membrane Protein on Ampicillin Resistance of Vibrio parahaemolyticus

Table 2

Primers used in this study.

NamesSequence (5′–3′)

Ptac-cre-FTTG​GAT​ACA​CCA​AGG​AAA​GTG​GTA​CCT​GAA​CGA​CCC​CGA​ATA​TTG​G
Ptac-cre-RACA​GAT​GTA​GGT​GTT​CCA​CAC​TGC​AGC​TAA​TCG​CCA​TCT​TCC​AGC​A
traJ-oriT-FCACT​AGTTCG​GGT​CGG​GTG​AAT​CTT
traJ-oriT-RACTTTCCTTGGTGTATCC
CmR-p15A-FTGT​GGA​ACA​CCT​ACA​TCT​G
CmR-p15A-RCACT​AGTATC​GTA​TGG​GGC​TGA​CTT
sacB-FCAG​CGC​TAG​CGG​AGT​GTA​TAC​CAC​CTT​TAT​GTT​GAT​AAG​AAA​TAA​AAG​AAA
sacB-RCAA​CAT​AGT​AAG​CCA​GTA​TAC​GGA​TCG​ATC​CTT​TTT​AAC​CCA​TC
GmR-RCGT​AAT​ACG​ACT​CAC​TAT​AGG​GC
GmR-FGCG​CAA​TTA​ACC​CTC​ACT​AAA​G
VP_RS22195-U-FAATCT​AGACAG​AAT​CAC​AGC​ACG​AGG
VP_RS22195-U-RGCC​CTA​TAG​TGA​GTC​GTA​TTA​CGC​TCC​CAC​AAC​AGT​CCA​CAT​AGC
VP_RS22195-D-FCTT​TAG​TGA​GGG​TTA​ATT​GCG​CCT​AAG​TAA​GAT​GTT​GTT​CCG​CG
VP_RS22195-D-RAATCT​AGATGG​CAG​CGA​ACA​AAG​GTG
VP_RS23020-U-FGGG​TTA​AAA​AGG​ATC​GAT​CCT​GTC​GTT​TTT​CGC​AAC​AAT​C
VP_RS23020-U-DGCC​CTA​TAG​TGA​GTC​GTA​TTA​CGA​AAA​TGG​CTT​GCT​CCC​GT
VP_RS23020-D-FCTT​TAG​TGA​GGG​TTA​ATT​GCG​CAG​CAC​GCA​TGT​AAC​CGT​G
VP_RS23020-D-RAAT​TTG​TGG​AAT​TCC​CGG​GAG​TAT​GAC​GTA​TAC​CCG​CAT​A
VP_RS16800-U-FAATCT​AGACCT​CTT​TTG​GCT​CGG​GTT
VP_RS16800-U-RGCC​CTA​TAG​TGA​GTC​GTA​TTA​CGA​AAC​GGG​CTA​ACT​ACT​GT
VP_RS16800-D-FCTT​TAG​TGA​GGG​TTA​ATT​GCG​CCG​TTA​CGG​ATG​TAT​GTG​T
VP_RS16800-D-RAATCT​AGAGAC​ACT​GAA​CTG​CCT​GTC
VP_RS16465-U-FAATCT​AGAAAG​ACG​AAC​CAG​GCA​AGG
VP_RS16465-U-RGCC​CTA​TAG​TGA​GTC​GTA​TTA​CGA​GTG​TCA​GCA​AGT​GCA​AC
VP_RS16465-D-FCTT​TAG​TGA​GGG​TTA​ATT​GCG​CCG​ATG​CTC​CGT​TTA​GTA​G
VP_RS16465-D-RAATCT​AGAGAA​TGG​CTA​GTA​AGG​AAT​GT
VP_RS20840-U-FAAAGTC​GACGAA​GAC​CGT​CAT​TTA​GCT
VP_RS20840-U-RGCC​CTA​TAG​TGA​GTC​GTA​TTA​CGG​CAA​GTC​TAT​GGG​GAA​GC
VP_RS20840-D-FCTT​TAG​TGA​GGG​TTA​ATT​GCG​CCA​TTC​TCA​TCA​AAA​GGG​G
VP_RS20840-D-RAAAGTC​GACGCC​ATA​CGG​AGG​ATT​CAT
VP_RS03765-U-FAATCT​AGAAAA​TCG​GTA​TGC​CAC​TTC
VP_RS03765-U-RGCC​CTA​TAG​TGA​GTC​GTA​TTA​CGG​CCG​CCT​AAT​AAC​ACT​GAC
VP_RS03765-D-FCTT​TAG​TGA​GGG​TTA​ATT​GCG​CGA​CCG​TAG​AAT​AGC​AGC​C
VP_RS03765-D-RAATCT​AGACAT​CGC​TCA​GTG​GGT​TT
VP_RS11205-U-FAATCT​AGACGC​CTC​TAT​CGT​TCG​CAT
VP_RS11205-U-RGCC​CTA​TAG​TGA​GTC​GTA​TTA​CGG​ACA​ATT​ACC​GGG​GGT​GAG
VP_RS11205-D-FCTT​TAG​TGA​GGG​TTA​ATT​GCG​CCC​TCT​TTA​GAA​AGT​TCT​ACC​G
VP_RS11205-D-RAATCT​AGATTC​AAC​ATC​TCT​TGG​ACT​C
RT-VP_RS22195-FCTA​AGC​AAC​CAA​GTT​AGC​CAA​C
RT-VP_RS22195-RTTC​TTC​TTG​AGC​AGC​CAT​TG
RT-VP_RS23020-FAGT​TGA​ATC​TCG​CCG​TAA​AT
RT-VP_RS23020-RCTGCTGGTTCTGCTTTCG
RT-VP_RS16800-FTGCTACATCGCCATCACG
RT-VP_RS16800-RGCA​TCA​ACT​ACC​GCT​TCT​TG
RT-VP_RS16465-FGCT​GGT​GAC​GAG​AAA​GAC​T
RT-VP_RS16465-RTTGAAGCCGATACCGAAG
RT-VP_RS20840-FCAA​CAG​GCT​CAC​TAG​GTG​CT
RT-VP_RS20840-RTTTGCGTCCATATCGTCC
RT-VP_RS03765-FCTC​AAA​CTA​TCG​GCA​CTG​GT
RT-VP_RS03765-RTTCGCTTGTGGGTGCTC
RT-VP_RS11205-FCGT​AAG​CGA​TTT​TCT​GTG​C
RT-VP_RS11205-RAAAGCGGCTGGGATTGG
RT-VP_RS17515-FGCT​TGT​CCG​TTT​GTG​TAT​CCC
RT-VP_RS17515-RTGC​TCA​ACT​GTT​AGT​TAC​GCC​TC
RT-VP_RS13510-FAAT​CAT​TGC​TCG​TTA​CCA​CAG
RT-VP_RS13510-RCCG​ACG​TAT​AGG​CTT​TCT​CTT​C
RT-mrcB-FGCGACAGAAGACCGAGAT
RT-mrcB-RCGT​TAA​GGT​ACT​GCC​ACC​T
RT-VP_RS02165-FTCGCTTACCGTGCCATC
RT-VP_RS02165-RTTT​TAC​ATC​CAG​CAT​CAC​CAC
RT-mrdA-FGTTTTGATGGGCTTGCTG
RT-mrd-RCCACTTTGATGCGGTTGT
RT-VP_RS21250-FTTGACGGGATGAAAACGG
RT-VP_RS21250-RAACAACTGCGATAAGGCG
RT-VP_RS22785-FTGGCATCCAAACCTCACC
RT-VP_RS22785-RCTT​GAA​GAC​TTT​CTC​GCT​GT
RT-dacB-FAGA​CCA​ACT​ATT​TCC​ACC​TG
RT-dacB-RAGTAGATAACGGCATCGG
RT-VP_RS22200-FACGCCACCTGCGTCTATT
RT-VP_RS22200-RCATTCCGATGCCGAAATC
RT-VP_RS09310-FTAA​TGA​CGG​CAC​TTA​TGG​T
RT-VP_RS09310-RACA​GAC​AAC​TGC​TCT​ACG​G
RT-VP_RS15980-FAGAAGAAGGCGACAACCG
RT-VP_RS15980-RGCACCGAGCGATAAAACG
RT-uhpA-FAAG​GCT​ATC​TAA​GCA​AAC​GC
RT-uhpA-RGCA​ATG​TCG​GAG​GTA​AGG​T
RT-VP_RS14060-FTAGAGCTCGGCGCAGATG
RT-VP_RS14060-RTTGCTTGCCCGGATACAG

Note. The restriction enzyme sites are in bold.