BioMed Research International / 2023 / Article / Tab 3 / Research Article
In Vitro Antibacterial and Phytochemical Screening of Hypericum perforatum Extract as Potential Antimicrobial Agents against Multi-Drug-Resistant (MDR) Strains of Clinical OriginTable 3 The constituent’s identification of the separated metabolites obtained from methanol extract of H. perforatum using LC/MS chromatographic separation technique.
Peak no. Retention time (min) Constituent identification Relative content (%) Molecular formula 1 6.40 L-Homocitrulline 5.20 C7 H15 N3 O3 188.9 2 8.60 Thiophene,2-[2-(3-methylphenyl)-ethenyl]- 2.71 C13 H12 S 200.1 3 8.99 2-Methyl-4-keto-pentan-2-ol 3.63 C11 H26 O 188.2 4 10.14 Butanoic acid 3.50 C10 H24 O3 88.41 5 10.71 Fumaric acid 3.86 C10 H20 O4 116.07 6 11.35 1,4-Butanediol 1.48 C10 H26 O2 90.12 7 11.73 Malic acid 3.32 C13 H30 O5 134.08 8 14.11 1,3,6,7-Tetrahydroxyxanthone 1.26 C13 H8 O6 259.2 9 15.76 Kaempferol 4.95 C15 H10 O6 287.05 10 16.95 Luteolin 1.34 C15 H10 O6 285.1 11 19.03 Erythritol 1.76 C16 H42 O4 122.12 12 21.34 Quercetin 2.71 C15 H10 O7 303.05 13 23.81 D-Fructofuranose 2.76 C21 H52 O6 179.16 14 24.42 Quercetin-3-O-α -L-arabinofuranoside 4.96 C20 H18 O11 434.3 15 25.87 D-Glucitol 2.33 C24 H62 O6 434.39 16 26.09 Quininic acid 1.97 C22 H52 O6 533.07 17 26.22 Procyanidin B2 1.08 C30 H26 O12 577.02 18 26.69 Myo-Inositol 2.42 C24 H60 O6 180.16 19 28.27 D-Allofuranose 1.04 C21 H52 O6 260.07 20 28.79 D-Gluconic acid 2.97 C24 H60 O7 629.24 21 29.30 D-Glucopyranose 1.23 C21 H52 O6 180.15 22 30.09 Gulonic acid, ç-lactone 1.58 C18 H42 O6 466.86 23 31.39 Nonadecanoate (ester) 0.61 C18 H42 O6 312.51 24 33.91 Palmitic acid 1.19 C19 H40 O2 255.34 25 37.40 1,3,4-Trihydro-2-thioxomethyl-2 h-isoquinoline 0.68 C10 H11 N 177.08 26 38.29 Stearic acid 0.73 C21 H44 O2 356.66 27 41.27 α -D-Glucopyranoside3.67 C36 H86 O11 341.11 28 42.21 Hypericin 10.89 C30 H16 O8 503.08 29 42.64 3-O-Coumaroyl-D-quinic acid 5.24 C31 H58 O8 699.21 30 43.32 Quercetin-3-β -D-galactopyranoside 8.45 C28 H24 O16 487.08 31 43.53 Deoxyfructosazine 1.83 C33 H76 N2 O7 304.30 32 44.05 Hyperforin 1.65 C35 H52 O4 536.8 33 45.24 Rutin 1.59 C27 H30 O16 611.16 34 46.18 Behenic acid 0.38 C25 H52 O2 340.62 35 46.81 Lactose 0.52 C36 H86 O11 918.02 36 48.55 D-(+)-Cellobiose 0.34 C37 H89 NO11 342.31 37 51.14 cis-5-O-Feruloylquinic acid 0.41 C32 H60 O9 729.2 38 53.56 Chlorogenic acid 1.62 C34 H66 O9 354.31 39 59.68 α -Amyrin = olean-12-en-3-ol0.53 C33 H58 O 426.72 40 60.75 Olean-12-en-3-ol, acetate 0.64 C32 H52 O2 468.8 41 63.78 Beta-sitosterol 0.87 C29 H50 O 576.83