In Vitro Antibacterial and Phytochemical Screening of Hypericum perforatum Extract as Potential Antimicrobial Agents against Multi-Drug-Resistant (MDR) Strains of Clinical Origin

Table 3

The constituent’s identification of the separated metabolites obtained from methanol extract of H. perforatum using LC/MS chromatographic separation technique.

Peak no.	Retention time (min)	Constituent identification	Relative content (%)	Molecular formula
1	6.40	L-Homocitrulline	5.20	C7H15N3O3	188.9
2	8.60	Thiophene,2-[2-(3-methylphenyl)-ethenyl]-	2.71	C13H12S	200.1
3	8.99	2-Methyl-4-keto-pentan-2-ol	3.63	C11H26O	188.2
4	10.14	Butanoic acid	3.50	C10H24O3	88.41
5	10.71	Fumaric acid	3.86	C10H20O4	116.07
6	11.35	1,4-Butanediol	1.48	C10H26O2	90.12
7	11.73	Malic acid	3.32	C13H30O5	134.08
8	14.11	1,3,6,7-Tetrahydroxyxanthone	1.26	C13H8O6	259.2
9	15.76	Kaempferol	4.95	C15H10O6	287.05
10	16.95	Luteolin	1.34	C15H10O6	285.1
11	19.03	Erythritol	1.76	C16H42O4	122.12
12	21.34	Quercetin	2.71	C15H10O7	303.05
13	23.81	D-Fructofuranose	2.76	C21H52O6	179.16
14	24.42	Quercetin-3-O-α-L-arabinofuranoside	4.96	C20H18O11	434.3
15	25.87	D-Glucitol	2.33	C24H62O6	434.39
16	26.09	Quininic acid	1.97	C22H52O6	533.07
17	26.22	Procyanidin B2	1.08	C30H26O12	577.02
18	26.69	Myo-Inositol	2.42	C24H60O6	180.16
19	28.27	D-Allofuranose	1.04	C21H52O6	260.07
20	28.79	D-Gluconic acid	2.97	C24H60O7	629.24
21	29.30	D-Glucopyranose	1.23	C21H52O6	180.15
22	30.09	Gulonic acid, ç-lactone	1.58	C18H42O6	466.86
23	31.39	Nonadecanoate (ester)	0.61	C18H42O6	312.51
24	33.91	Palmitic acid	1.19	C19H40O2	255.34
25	37.40	1,3,4-Trihydro-2-thioxomethyl-2 h-isoquinoline	0.68	C10H11N	177.08
26	38.29	Stearic acid	0.73	C21H44O2	356.66
27	41.27	α-D-Glucopyranoside	3.67	C36H86O11	341.11
28	42.21	Hypericin	10.89	C30H16O8	503.08
29	42.64	3-O-Coumaroyl-D-quinic acid	5.24	C31H58O8	699.21
30	43.32	Quercetin-3-β-D-galactopyranoside	8.45	C28H24O16	487.08
31	43.53	Deoxyfructosazine	1.83	C33H76N2O7	304.30
32	44.05	Hyperforin	1.65	C35H52O4	536.8
33	45.24	Rutin	1.59	C27H30O16	611.16
34	46.18	Behenic acid	0.38	C25H52O2	340.62
35	46.81	Lactose	0.52	C36H86O11	918.02
36	48.55	D-(+)-Cellobiose	0.34	C37H89NO11	342.31
37	51.14	cis-5-O-Feruloylquinic acid	0.41	C32H60O9	729.2
38	53.56	Chlorogenic acid	1.62	C34H66O9	354.31
39	59.68	α-Amyrin = olean-12-en-3-ol	0.53	C33H58O	426.72
40	60.75	Olean-12-en-3-ol, acetate	0.64	C32H52O2	468.8
41	63.78	Beta-sitosterol	0.87	C29H50O	576.83