High Expression of SMO and GLI1 Genes with Poor Prognosis in Malignant Mesothelioma

Table 1

Retrotranscriptional response system and reaction conditions.

The reverse transcription system	Reaction conditions
DNA digester buffer (2 ul)	42°C	2 min
gDNA digester (1 ul)	42°C	2 min
Template RNA (1 ul)	42°C	2 min
RNase-free H2O (6 ul)	42°C	2 min
2XHifair II SuperMix plus (10 ul)	25°C	5 min
	42°C	30 min
	85°C	5 min