Rapid Detection of Beta-Lactamases Genes among Enterobacterales in Urine Samples by Using Real-Time PCR

Table 1

List of primers used for qPCR amplification of ESBLs and carbapenemase genes.

Genes	Primer sequence (5→3); FW: Forward; RV: Reverse	T°m	Product size (bp)	GenBank
blaSHV-12	FW: AGCCGCTTGAGCAAATTAAA	59.99	77	LC229232.1
	RV: GCTGGCCAGATCCATTTCTA	60.18
blaTEM	FW: GATAAATCTGGAGCCGGTGA	60.04	78	MG860488.1
	RV: GATACGGGAGGGCTTACCAT	60.17
blaCTX-M-15	FW: CACCAATGATATTGCGGTGA	60.34	77	MG288677.1
	RV: GTTGCGGCTGGGTAAAATAG	59.61
blaCTX-M-9	FW: TACTTCACCCAGCCTCAACC	60.11	78	CP028990.1
	RV: ACCGTCGGTGACGATTTTAG	59.99
blaCMY-2	FW: CCAGAACTGACAGGCAAACA	59.87	65	LC229227
	RV: CCTGCCGTATAGGTGGCTAA	60.11
blaOXA-48	FW: GTAGTCAGCGCATCGTGAAA	60.02	73	MN654469.1
	RV: CCCGTTTTAGCCCGAATAAT	60.12
blaIMP-1	FW: GCCAAAGTCCGCCAAATTAT	60.17	92	MK088089.1
	RV: TCAAGAGTGATGCGTCTCCA	65.56
blaNDM-1	FW: ATGGAGACTGGCGACCAAC	61.10	87	LC413788.2
	RV: GGCATGTCGAGATAGGAAGG	59.65