Production Optimization and Biochemical Characterization of Cellulase from Geobacillus sp. KP43 Isolated from Hot Spring Water of Nepal

Table 2

Activity of cellulase enzyme at different purification steps.

Enzyme	Activity (U/mL)	Protein content (mg/mL)	Specific activity (U/mg)	Purification fold
Crude	0.010	0.051	0.186	1
Salt precipitated and dialyzed	0.024	0.128	0.190	1.021
Column purified	0.026	0.055	0.467	2.488