Antimicrobial Resistance, Multilocus Sequence, and spa Typing of Staphylococcus aureus Isolated from Retail Raw Meat Products

Table 2

Antimicrobial resistance, spa typing, and MLST analysis of S. aureus isolates from retail raw meat products.

SI no	Isolate^a	Methicillin resistance^b	Phenotypic resistance profile^c	Genotypic resistance profile	spa typing		MLST^f
					spa type	Repeats	Allelic profile	ST (CC)
1	G1	MSSA	PEN, SUL	blaZ, norA, grlA, sulI	t005	26-23-13-23-31-05-17-25-17-25-16-28
2	G2	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t279	07-23-12-34-34-34-12-12-23-02-12-23
3	G3	MSSA	PEN, SUL	blaZ, norA, grlA, sulI	t1677	11-19-12-12-17-34-24-34-22-25
4	G4	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t1677	11-19-12-12-17-34-24-34-22-25
5	G5	MSSA	PEN, TET, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t091	07-23-21-17-34-12-23-02-12-23	5-4-1-4-4-6-3	7
6	G6	MSSA	PEN, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t1677	11-19-12-12-17-34-24-34-22-25
7	G7	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t359	07-23-12-21-17-34-34-33-34
8	G8	MSSA	PEN, TET, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t091	07-23-21-17-34-12-23-02-12-23	5-4-1-4-4-6-3	7
9	G9	MSSA	PEN, SUL	blaZ, tetK, grlA, sulI	t1677	11-19-12-12-17-34-24-34-22-25
10	G10	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t005	26-23-13-23-31-05-17-25-17-25-16-28
11	G11	MSSA	PEN, TET, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t091	07-23-21-17-34-12-23-02-12-23	5-4-1-4-4-6-3	7
12	G12	MSSA	PEN, TET, SUL	blaZ, tetK, norA, grlA, sulI	t091	07-23-21-17-34-12-23-02-12-23	5-4-1-4-4-6-3	7
13	G13	MSSA	PEN, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t346	07-23-12-34-12-12-23-02-12-23
14	G14	MSSA	PEN, TET, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t084	07-23-12-34-34-12-12-23-02-12-23	13-13-1-1-12-11-13	15 (15)
15	G15	MSSA	PEN, TET, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t279	07-23-12-34-34-34-12-12-23-02-12-23	13-13-1-1-12-1-13	199 (15)
16	G16	MSSA	PEN, SUL	blaZ, chlA, tetK, grlA, sulI	t091	07-23-21-17-34-12-23-02-12-23
17	G17	MSSA	PEN, TET, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t279	07-23-12-34-34-34-12-12-23-02-12-23	13-13-1-1-12-1-13	199 (15)
18	C1	MSSA	PEN, SUL	blaZ, chlA, tetK, tetM, sulI	t1451	08-16-02-25-34-25
19	C2	MSSA	PEN, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t127	07-23-21-16-34-33-13
20	C3	MSSA	PEN, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t1201	07-16-34-34-33-34
21	C4	MSSA	PEN, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t091	07-23-21-17-34-12-23-02-12-23
22	C5	MRSA	PEN, GEN, CHL^d, TET, ERY, CIP, TMP, SUL	blaZ, aacA-aphD, chlA, tetK, tetM, ermA, norA, grlA, sulI	t005	26-23-13-23-31-05-17-25-17-25-16-28	3-31-1-1-4-4-3	7435 (8)
23	C6	MSSA	PEN, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t267	07-23-12-21-17-34-34-34-33-34
24	C7	MSSA	PEN, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t091	07-23-21-17-34-12-23-02-12-23
25	C8	MSSA	PEN, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t267	07-23-12-21-17-34-34-34-33-34
26	C9	MSSA	PEN, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t091	07-23-21-17-34-12-23-02-12-23
27	C10	MRSA	PEN, TET, SUL	blaZ, chlA, tetK, tetM, sulI	t9428	07-23-13-23-31-05-17-25-17-25	5-4-1-4-4-6-3	7
28	C11	MSSA	PEN, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t267	07-23-12-21-17-34-34-34-33-34
29	C12	MRSA	PEN, TET, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t7258	15-19-12-21-17-34-24-34-22-25	5-4-1-4-4-6-3	7
30	C13	MRSA	PEN, TET, ERY^d, SUL	blaZ, chlA, tetK, tetM, norA, sulI	t852	07-23-13-23-31-05-17-25-17-25-16-28	13-4-1-4-4-287-13	7436^g
31	S1	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t1234	07-23-12-12-34-34-34-33-34
32	S2	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t14538	26-23-13-23-31-05-23-31-05-17-25-17-25-16-28
33	S3	MSSA	PEN, TET^d SUL	blaZ, norA, grlA, sulI	t189	07-23-12-21-17-34
34	S4	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t14538	26-23-13-23-31-05-23-31-05-17-25-17-25-16-28
35	S5	MSSA	PEN, ERY^d, SUL	blaZ, norA, grlA, sulI	t14538	26-23-13-23-31-05-23-31-05-17-25-17-25-16-28
36	S6	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t19851^e	07-23-12-12-34-12-12-23-20-12-23	13-13-1-1-12-11-13	15 (15)
37	S7	MSSA	PEN, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t6099	07-23-12-21-17-34-33-16-16-16-23
38	S8	MSSA	PEN, CHL^d, SUL	blaZ, tetK, norA, grlA, sulI	t008	11-19-12-21-17-34-24-34-22-25
39	S9	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t786	07-12-21-17-13-34-34-33-34
40	S10	MSSA	PEN, SUL	blaZ, chlA, norA, grlA, sulI	t786	07-12-21-17-13-34-34-33-34
41	S11	MSSA	PEN, ERY, SUL	blaZ, tetK, norA, grlA, sulI	t008	11-19-12-21-17-34-24-34-22-25	3-35-19-2-20-26-39	398
42	S12	MSSA	PEN, TET, SUL	blaZ, chlA, tetK, tetM, norA, grlA, sulI	t346	07-23-12-34-12-12-23-02-12-23	13-13-1-444-12-11-13	5585 (15)
43	F1	MSSA	PEN, SUL	blaZ, chlA, norA, grlA, sulI	t1234	07-23-12-12-34-34-34-33-34
44	F2	MSSA	PEN, SUL	blaZ, chlA, norA, grlA, sulI	t6099	07-23-12-21-17-34-33-16-16-16-23
45	F3	MSSA	PEN, SUL	blaZ, chlA, tetK, norA, grlA, sulI	t14538	26-23-13-23-31-05-23-31-05-17-25-17-25-16-28
46	F4	MSSA	PEN, ERY^d, SUL	blaZ, norA, grlA, sulI	t19851^e	07-23-12-12-34-12-12-23-20-12-23	13-13-1-1-12-11-13	15 (15)
47	F5	MSSA	PEN, ERY^d, SUL	blaZ, chlA, norA, grlA, sulI	t1875	07-23-12-34-12-23
48	F6	MSSA	PEN, SUL	blaZ, tetK, norA, grlA, sulI	t008	11-19-12-21-17-34-24-34-22-25
49	F7	MSSA	PEN, SUL	blaZ, chlA, norA, grlA, sulI	t786	07-12-21-17-13-34-34-33-34
50	F8	MSSA	PEN, ERY^d, SUL	blaZ, tetK, norA, grlA, sulI	t1875	07-23-12-34-12-23

^aG: ground beef; C: chicken meat; S: seawater fish; F: freshwater fish. ^bMSSA: methicillin-sensitive S. aureus; MRSA: methicillin-resistant S. aureus harboring the mecA gene. ^cPEN: penicillin G; SUL: sulphamethoxazole; ERY: erythromycin; TET: tetracycline; CHL: chloramphenicol; GEN: gentamicin; CIP: ciprofloxacin; TMP: trimethoprim. ^dIntermediate resistance to the indicated antimicrobial agent according to CLSI standards. ^eNew spa types are shown in bold font. ^fMultilocus sequence typing (MLST) was conducted for 13 multidrug-resistant isolates (three or more antimicrobial classes) and for two isolates belonging to a new spa type. ST: sequence type; CC: clonal complex. ^gNew STs types are identified by bold type.