Next-Generation Probiotics: Microflora Intervention to Human Diseases

Table 2

Culturing approaches to NGPs and Candidatus species.

Reference	Bacteria species/strain	Culture media	Disease target
[39]	Akkermansia muciniphila ATCCBAA-835	BHI, soft agar mucin medium	Type 2 diabetes and weight controls
[61]	Faecalibacterium prausnitzii ATCC 27766	YCFA	Chronic moderate and severe colitis
[83]	Bacteroides fragilis ZY-312	BHI	Necrotizing enterocolitis
[108]	Eubacterium hallii DSM 3353	YCFA and contained approximately 33 mM acetate	Balance of intestinal microflora
[109]	Roseburia intestinalis DSMZ-14610	Composition of medium: processed water 40 ml, soybean-casein, digest broth 2.75%, yeast extract 0.2%, animal tissue digest 0.05%, dextrose 0.2%, hemin 0.0005%, menadione 0.00005%, sodium citrate 0.02%, thiols 0.1%, sodium pyruvate 0.1%, saponin 0.26%, antifoaming agent 0.01%, sodium polyanetholsulfonate 0.035%	Crohn’s disease

YCFA: yeast-extract-casein hydrolysate-fatty acids; BHI: brain–heart infusion.