Research Article
[Retracted] NORAD Promotes the Viability, Migration, and Phenotypic Switch of Human Vascular Smooth Muscle Cells during Aortic Dissection via LIN28B-Mediated TGF-β Promotion and Subsequent Enhanced Glycolysis
Figure 3
NORAD recruited LIN28B to stabilize TGF-β mRNA. (a) Subcellular fractionation test was performed to confirm the nuclear-cytoplasmic fractionation of NORAD in VSMCs. (b) Effect of NORAD silence on the mRNA expression of PDGF, VEGF, FGF, and TGF-β. (c) Effect of NORAD silence on the protein TGF-β level. (d) The interreaction of NORAD and RNA-binding protein was verified by RIP. (e) The impact of NORAD silencing on the complex of LIN28B to TGF-β was evaluated by the RIP assay. (f) The mRNA stabilization of TGF-β was detected by qRT-PCR. Data were presented as the . was shown in comparison to the negative control.
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