Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA₂s

Figure 5

LED photobiomodulation effect on LDH liberation by TG-macrophages under Bothrops jararacussu snake venom and bothropstoxins stimulation. Thioglycollate-elicited murine macrophages were isolated from peritoneal cavity after 96 h of thioglycollate injection. 2x10⁵ cells were incubated for 30 min with the inhibitors apocynin (350 nM) or CCCP 3 (240 nM) followed by 90 min of stimulation of cells with Hanks (control), PMA (500 ng/mL; positive control), Bothrops jararacussu venom, or bothropstoxins (25 μg/mL) under LED irradiation or without (non LED) at 37°C in a humid atmosphere of 5% CO₂. LDH liberation was quantified by spectrophotometric absorbance measurement at 340 nm. The results were expressed in absorbance and represent the mean ± S.E.M. of 5 animals. in comparison to control group (RPMI) and in comparison to the respective control group (ANOVA).