Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA₂s

Figure 3

LED photobiomodulation effect on peroxidase activity by TG-macrophages under Bothrops jararacussu snake venom and bothropstoxins stimulation. Thioglycollate-elicited murine macrophages were isolated from peritoneal cavity after 96 h of thioglycollate injection. 2x10⁵ cells were incubated for 90 min with RPMI (control), Bothrops jararacussu venom, or bothropstoxins (25 μg/mL) under LED irradiation or without (non LED) at 37°C in a humid atmosphere of 5% CO₂. Peroxidase activity was quantified by spectrophotometric fluorescence measurement at excitation/emission 535/587 nm. The results were expressed in mU/mL of peroxidase activity and represent the mean ± S.E.M. of 5 animals. in comparison to control group (RPMI) and in comparison to the respective control group (ANOVA).