Research Article

Transcriptome Analysis of the Accumulation of Astaxanthin in Haematococcus pluvialis Treated with White and Blue Lights as well as Salicylic Acid

Table 1

Primers used for the quantitative real-time PCR (qRT-PCR) analysis of four genes involved in the biosynthetic pathway of astaxanthin in Haematococcus pluvialis. Symbols “F” and “R” stand for upstream and downstream primers, respectively.

Gene (GenBank accession)Primer and sequence

actin encoding actin of H. lacustris (GFH15240.1)actinF: AGCGGGAGATAGTGCGGGACA
actinR: ATGCCCACCGCCTCCATGC
bkt encoding beta-carotene ketolase (GU143688.1)bktF: AGCCACCACCTTCCGACACAA
bktR: ACGACGACGATGTGCATCAGAC
crtZ encoding carotenoid hydroxylase (AY187011.1)crtZF: CCAGCAGAGGCATCGACACATC
crtZR: AGGCGAGAGTGACCACTGAGAC
lyc encoding lycopene beta cyclase (KX424526.1)lycF: TCTGGACGCACGCCTCAAGT
lycR: GGCAGTTCAGGTGGCAGTTCAG
psy encoding phytoene synthase (AY835634.1)psyF: CTCCCGCCATGCTTCACCAATC
psyR: GGCAATCGGCGATGCAAGTCA