Harnessing Mucor spp. for Xylanase Production: Statistical Optimization in Submerged Fermentation Using Agro-Industrial Wastes

Table 3

Box–Behnken design of optimization variables with experimental and predicted xylanase activity of both M. circinelloides and M. hiemalis.

Run order	Variables					Xylanase activity (U/mL)
						M. circinelloides		M. hiemalis
	A	B	C	D	E	Experimental response	Predicted response	Experimental response	Predicted response
1	40	7	7	5	3	17.73	18.54	17.32	17.49
2	30	7	7	5	1	27.63	27.49	17.23	18.32
3	40	7	7	3	1	31.92	31.24	28.70	28.42
4	20	7	7	3	1	28.62	28.03	30.02	28.82
5	20	9	7	3	3	38.52	38.49	29.69	31.58
6	40	9	7	3	3	23.42	23.39	24.00	24.96
7	30	9	7	3	1	31.67	33.36	28.45	29.76
8	30	9	5	3	3	23.26	22.76	25.07	25.48
9	30	7	7	3	3	27.38	28.56	19.79	23.78
10	30	5	9	3	3	28.45	27.53	32.91	32.16
11	30	5	7	5	3	27.22	26.26	28.12	27.07
12	30	5	7	3	1	32.58	33.81	26.89	27.84
13	30	7	5	3	1	28.37	28.36	27.79	26.20
14	30	9	7	3	5	34.72	34.49	19.54	19.21
15	40	7	7	3	5	15.50	14.68	16.90	16.68
16	30	7	7	3	3	29.28	28.56	23.50	23.78
17	30	5	5	3	3	31.84	30.84	25.32	26.07
18	40	7	5	3	3	25.24	24.05	26.72	26.33
19	20	5	7	3	3	30.02	31.23	34.89	36.26
20	20	7	7	1	3	30.27	29.44	30.93	29.60
21	30	7	9	3	5	33.40	33.82	24.08	24.25
22	30	7	9	3	1	34.81	33.59	26.80	24.94
23	30	7	9	1	3	25.81	25.87	21.11	22.74
24	30	5	7	3	5	28.54	27.85	29.61	28.92
25	30	7	5	3	5	21.69	23.31	16.99	17.42
26	30	9	7	5	3	30.10	29.43	24.49	22.92
27	30	9	9	3	3	42.23	41.80	26.06	24.96
28	30	7	7	3	3	26.89	28.56	23.17	23.78
29	30	7	9	5	3	32.83	32.45	28.78	28.29
30	30	5	7	1	3	26.39	26.31	32.58	31.54
31	30	7	7	1	5	24.91	25.05	17.15	18.30
32	40	5	7	3	3	23.26	24.46	27.63	28.08
33	30	9	7	1	3	29.11	29.32	29.44	27.88
34	20	7	7	5	3	28.95	29.26	30.85	32.15
35	30	7	7	3	3	28.21	28.56	25.40	23.78
36	30	7	7	1	1	28.95	28.66	31.59	33.18
37	40	7	7	1	3	18.64	18.30	31.92	29.46
38	30	7	7	5	5	25.98	26.28	23.09	23.74
39	30	7	5	1	3	23.42	24.56	28.21	30.22
40	30	7	7	3	3	29.86	28.56	25.48	23.78
41	20	7	7	3	5	40.50	39.76	32.25	31.10
42	20	7	5	3	3	22.43	21.66	26.39	24.86
43	40	7	9	3	3	17.56	18.59	18.47	20.24
44	30	7	7	3	3	29.77	28.56	25.32	23.78
45	30	7	5	5	3	17.32	18.03	15.34	15.24
46	20	7	9	3	3	41.41	42.85	35.88	36.52