Detection of Campylobacter jejuni Based on a Real-Time Fluorescence Loop-Mediated Isothermal Amplification Method

Figure 2

Analytical sensitivity of the LAMP and PCR assay in detecting C. jejuni. (a) The sensitivity of the LAMP assay was monitored by a real-time PCR system using serial dilutions of the C. jejuni DNA template. NC: negative control. (b) Melting peaks were generated by melting curve analysis. (c) Lanes 1-9: the concentrations used as templates were  ng/μL, respectively. M: DL2000 marker. (d) The detection results of the PCR. M: DL500 marker.

(a)

(b)

(c)

(d)