Detection of Campylobacter jejuni Based on a Real-Time Fluorescence Loop-Mediated Isothermal Amplification Method

Figure 1

Analytical specificity of the LAMP and PCR for detecting C. jejuni. (a) The amplification reaction of 74 bacterial strains was monitored by a real-time PCR System. (b) The result of the LAMP assay was identified with naked eyes. White magnesium pyrophosphate was visualized in the positive amplification (15-18), while no precipitate was found in the negative amplifications (5-8). (c, d) Analytical specificity evaluation of the LAMP assay (c) and conventional PCR (d) by electrophoresis. M: DL2000 marker; M: DL500 marker (Takara). 15: C. jejuni, ATCC33252; 16: C. jejuni, ATCCBAA-1153; 17: C. jejuni, ATCC33291; 18: C. jejuni, ATCC33560. Others were the non-C. jejuni bacterial strains and negative control (NC) which are listed in Table 1.

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