Computer Image Analysis Reveals C-Myc as a Potential Biomarker for Discriminating between Keratoacanthoma and Cutaneous Squamous Cell Carcinoma

Figure 7

Expression and analysis of Ki-67 in Keratoacanthoma (KA) and Cutaneous Squamous Cell Carcinoma (cSCC). (a-1) Immunohistochemical staining. Ki-67 in KA samples was located in the initial portion of tumor infiltration, whereas in cSCC samples, there was diffuse nuclear staining (20x). (a-2) Tissue area delineation (red: tumor target area; green: mesenchyme; and blue: blank). (a-3) Cytoarchitectonic delineation (green). (a-4) Ki-67 DAB Score delineation (Score 0: blue; Score 1+: yellow; Score 2+: orange; and Score 3+: dark red). (b) Percent of Scores 0, 1+, 2+, and 3+ in grades of KA and cSCC samples based on differences in Ki-67 DAB positive intensities, with Ki-67 Score 0 (), Ki-67 Score 1+ (), Ki-67 Score 2+ (), and Ki-67 Score 3+ (). (c) -Scores in KA and cSCC samples based on differences in intensities of Ki-67 DAB positivity (). (d) Cross-comparisons of the Ki-67 Score among the three subtypes of KA (KA-well developed, KA-cSCC, KA-regressing) were not statistically significant.

(a)

(b)

(c)

(d)