Computer Image Analysis Reveals C-Myc as a Potential Biomarker for Discriminating between Keratoacanthoma and Cutaneous Squamous Cell Carcinoma

Figure 6

Expression and analysis of CyclinD1 in Keratoacanthoma (KA) and Cutaneous Squamous Cell Carcinoma (cSCC). (a-1) Immunohistochemical staining. CyclinD1 in KA samples was located in the initial portion of tumor infiltration, whereas in cSCC samples, there was diffuse nuclear staining (20x). (a-2) Tissue area delineation (red: tumor target area; green: mesenchyme; and blue: blank). (a-3) Cytoarchitectonic delineation (green). (a-4) CyclinD1 DAB Score delineation (Score 0: blue; Score 1+: yellow; Score 2+: orange; and Score 3+: deep red). (b) Percent of Scores 0, 1+, 2+, and 3+ in classes of KA and cSCC samples based on CyclinD1 DAB positive intensity, with CyclinD1 Score 1+ (). (c) -Scores in KA versus cSCC samples based on different intensities of CyclinD1 DAB positivity (). (d) Cross-comparisons of the CyclinD1 Score among the three subtypes of KA (KA-well developed, KA-cSCC, KA-regressing) were not statistically significant.

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