Computer Image Analysis Reveals C-Myc as a Potential Biomarker for Discriminating between Keratoacanthoma and Cutaneous Squamous Cell Carcinoma

Figure 5

Expression and analysis of C-Myc in Keratoacanthoma (KA) and Cutaneous Squamous Cell Carcinoma (cSCC). (a-1) Immunohistochemical staining. C-Myc in KA samples was located in the initial portion of tumor infiltration, whereas in cSCC samples, there was diffuse nuclear staining (20x). (a-2) Tissue area delineation (red: tumor target area; green: mesenchyme; and blue: blank). (a-3) Cytoarchitectonic delineation (green). (a-4) C-Myc DAB Score delineation (Score 0: blue; Score 1+: yellow; Score 2+: orange; and Score 3+: dark red). (b) Expression of C-Myc in KA and cSCC patients with arrows indicating the basal region of KA tumors. (c) Percent Scores 0, 1+, 2+, and 3+ in grades of KA versus cSCC samples based on the intensity of C-Myc DAB positivity, with C-Myc Score 0 () and C-Myc Score 2+ (). (d) C-Myc -Scores in KA versus cSCC based on different intensities of C-Myc DAB positivity (). (e) Cross-comparisons of the C-Myc Score among the three subtypes of KA (KA-well developed, KA-cSCC, KA-regressing) were not statistically significant.

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