Physiological Response of the Freshwater Mussel Unio douglasiae in Microcystis aeruginosa Bloom Waters

Table 1

Experimental design for the freshwater mussel Unio douglasiae. Filtration rate (FR) and biodeposition (BD) as faeces and pseudofaeces of U. douglasiae under different conditions over 7 hours of cultivation.

Variables	Mussel size	Mussel density	Type of prey	Concentration of prey	Temperature	Dissolved oxygen	Water depth
Shellfish tested/replicates	19/1	3/3	2/3	7/3	4/3	3/3	3/3
Volume (L)	2.0	2.0	2.0	2.0	9.0	2.0	57.0
Mussel size (cm)	4.2–9.8	6.3–9.1	6.3–9.1/5.5–8.5	7.3–9.2	5.0–7.9	6.5–7.1	6.5–8.9
Mussel density (individuals·L^-1)	0.5	0.3, 1.0, 2.0	1.0	0.7	0.3	1.5	0.1
Type of prey	ABW	ABW	RW/LW	ABW	ABW	ABW	ABW
Concentration of prey (chl-a, μg·L^-1)	138.9	138.9	47.1/25.4	49.4, 113.2, 199.7, 265.7, 327.4, 409.81	49.4	190.0	87.8
Water temperature (°C)	23	23	1.4/4.3	23	5, 15, 25, 35	23	21.5
Dissolved oxygen (mg L^-1)	7–8	7–8	10–12/8–9	7–8	7–8	0.5, 4.5, 9.0	8.5–8.1
Water depth (cm)	7–9	7–9	7–9	7–9	10–12	7–9	20, 50, 80
pH	8.3	8.3	8.4/8.1	8.3–9.8	8.5	8.3–8.6	8.2