HIF1<i>α</i> Promotes BMP9-Mediated Osteoblastic Differentiation and Vascularization by Interacting with CBFA1

<div><i>In vivo</i> effects of the PLGA-transfected iMEF composite on vessel formation upon subcutaneous implantation into rats. (a) Schematic diagram showing the transfected iMEFs seeded on electrospun PLGA scaffolds implanted into the dorsal subcutaneous space of the mice. (b) Scanning electron microscopy image of the PLGA scaffold (b1) and seeded cells (b2) on the PLGA scaffold (<span class="nowrap"><svg height="12.7112pt" id="M24" style="vertical-align:-3.403299pt" version="1.1" viewbox="-0.0498162 -9.3079 118.552 12.7112" width="118.552pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.608,0)"><path d="M433 39L423 65C413 59 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(d) Immunofluorescence staining for CD31 (vascular formation displays green fluorescence) and the average number of vascular structures in a high-power field for each implant (<span class="nowrap"><svg height="12.7373pt" id="M25" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -9.3079 92.068 12.7373" width="92.068pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M319 325C317 349 306 409 297 431C277 440 250 449 209 449C117 449 57 389 57 319C57 243 122 209 182 182C232 159 261 135 261 91C261 48 227 21 190 21C130 21 85 79 68 145L41 140C41 104 51 36 58 22C75 7 121 -12 172 -12C252 -12 337 35 337 126C337 195 286 231 210 262C166 281 126 304 126 348C126 388 152 417 191 417C240 417 274 378 294 318L319 325Z"></path></g><g transform="matrix(.013,0,0,-0.013,4.875,0)"><path d="M390 111C344 68 312 56 269 56C212 56 118 102 118 241C118 346 175 401 241 401C277 401 312 388 342 360C350 352 355 349 361 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BioMed Research International

fig5

Figure 5

Figure 5: HIF1<i>α</i> Promotes BMP9-Mediated Osteoblastic Differentiation and Vascularization by Interacting with CBFA1 

BioMed Research International

HIF1α Promotes BMP9-Mediated Osteoblastic Differentiation and Vascularization by Interacting with CBFA1

Figure 5