[Retracted] Targeting the lncRNA FGD5-AS1/miR-497-5p/PD-L1 Axis Inhibits Malignant Phenotypes in Colon Cancer (CC)

Figure 4

lncRNA FGD5-AS1 sponged miR-497-5p to upregulate PD-L1 in CC cells. The online starBase software (http://starbase.sysu.edu.cn/) was used to predict the binding sites of miR-497-5p with (a) lncRNA FGD5-AS1 and (e) 3UTR of PD-L1 mRNA. The binding sites of miR-497-5p with (b–d) lncRNA FGD5-AS1 and (f–h) 3UTR of PD-L1 mRNA were validated by using the dual-luciferase reporter gene system assay. MiR-497-5p negatively regulated PD-L1 at (i) transcriptional and (j) translated levels. (k, l) Upregulation of lncRNA FGD5-AS1 promoted PD-L1 expressions through downregulating miR-497-5p. Each experiment repeated at least 3 times, and “” represented .

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