Prevalence and Antimicrobial Resistance of Campylobacter Species in Diarrheal Patients in Mymensingh, Bangladesh

Table 1

Primers and conditions used for different PCR assays.

Primers	Sequence (5-3)	Target/purpose	Amplicon size (bp)	PCR condition (30 cycle)			Reference
				Denaturation	Annealing	Extension
16S9F; 16S1540R	GAGTTTGATCCTGGCTC; AAGGAGGTGATCCAGCC	16S rRNA	1530	94°C, 30 s	47°C, 30 s	72°C, 90 s	[34]
Cj-CdtAU2; Cj-CdtAR2	AGGACTTGAACCTACTTTTC; AGGTGGAGTAGTTAAAAACC	CjcdtA	631	94°C, 30 s	53°C, 30 s	72°C, 30 s	[35]
Cc-CdtAU1; Cc-CdtAR1	ATTGCCAAGGCTAAAATCTC; GATAAAGTCTCCAAAACTGC	CccdtA	329
Cf-CdtCU2; Cf-CdtCR2	AACGACAAATGTAAGCACTC; TATTTATGCAAGTCGTGCGA	CfcdtA	489
HIP400F; HIP1134R	GAAGAGGGTTTGGGTGGTG; AGCTAGCTTCGCATAATAACTTG	hipO gene	735	94°C, 30 s	55°C, 30 s	72°C, 45 s	[36]