Development of Microsatellite Marker System to Determine the Genetic Diversity of Experimental Chicken, Duck, Goose, and Pigeon Populations

Table 3

Number of alleles, optimal amplification conditions, and fragment length of 14 alleles for the outbred colony laboratory geese.

Loci	Primer sequence(5-3)	Temperature (°C)	Allele range
G-Ans17	ACAAATAACTGGTTCTAAGCAC	51.0	111–123
	AGAGGACTTCTATTCATAAATA
G-TTUCG1	CCCTGCTGGTATACCTGA	53.0	113-115
	GTGTCTACACAACAGC
G-APH13	CAACGAGTGACAATGATAAAA	53.0	163-165
	CAATGATCTCACTCCCAATAG
G-Ans02	TTCTGTGCAGGGGCGAGTT	58.0	202–230
	AGGGAACCGATCACGACATG
G-Ans07	GACTGAGGAACTACAATTGACT	62.0	236–246
	ACAAAGACTACTACTGCCAAG
G-Ans18	GTGTTCTCTGTTTATGATATTAC	56.0	229–237
	AACAGAATTTGCTTGAAACTGC
G-Ans25	CACTTATTAATGGCACTTGAAA	62.0	261–277
	GTTCTCTTGTCACAACTGGA
G-Hhiμ1b	ATCAAAGGCACAATGTGAAAT	60.0	212–216
	AGTAAGGGGGCTTCCACC
G-CKW47	AACTTCTGCACCTAAAAACTGTCA	56.0	213-215
	TGCTGAGGTAACAGGAATTAAAA
G-Bcaμ5	AGTGTTTCTTTCATCTCCACAAGC	56.0	197-201
	AGACCACAATCGGACCACATATTC
G-Bcaμ7	TAGTTTCTATTTGCACCCAATGGAG	60.0	171-175
	CGGTCCTGTCCTTGTGCTGTAA
G-Bcaμ8	CCCAAGACTCACAAAACCAGAAAT	58.0	155-159
	ATGAAAGAAGAGTTAAACGTGTGCAA
G-CAUD006	ATGGTTCTCTGTAGGCAATC	56.0	170-210
	TTCTGCTTGGGCTCTTGGA
G-APH20	ACCAGCCTAGCAAGCACTGT	53.0	140-150
	GAGGCTTTAGGAGAGATTGAAAAA