Development of Microsatellite Marker System to Determine the Genetic Diversity of Experimental Chicken, Duck, Goose, and Pigeon Populations

Table 2

Number of alleles, optimal amplification conditions, and fragment length of 28 alleles for the laboratory ducks.

Loci	Primer sequence(5-3)	Temperature (°C)	Allele range	Applicable groups
CAUD007	ACTTCTCTTGTAGGCATGTCA	60.8	100-190	Outbred group
	CACCTGTTGCTCCTGCTGT
CAUD004	TCCACTTGGTAGACCTTGAG	60.8	234-385	Outbred group
	TGGGATTCAGTGAGAAGCCT
CAUD023	CACATTAACTACATTTCGGTCT	51.4	163-234	Outbred group
	CAGCCAAAGAGTTCAACAGG
CAUD027	AGAAGGCAGGCAAATCAGAG	66.0	70-180	Outbred group
	TCCACTCATAAAAACACCCACA
CAUD001	ACAGCTTCAGCAGACTTAGA	55.5	150-247	Outbred group
	GCAGAAAGTGTATTAAGGAAG
CAUD031	AGCATCTGGACTTTTTCTGGA	51.4	107-187	Outbred group
	CACCCCAGGCTCTGAGATAA
CAUD032	GAAACCAACTGAAAACGGGC	58.1	96-206	Outbred group
	CCTCCTGCGTCCCAATAAG
AY314	CTCATTCCAATTCCTCTGTA	50.3	112-329	Outbred group
	CAGCATTATTATTTCAGAAGG
CMO211	GGATGTTGCCCCACATATTT	55.0	112-205	Outbred group
	TTGCCTTGTTTATGAGCCATT
APH09	GGATGTTGCCCCACATATTT	58.0	134-190	Outbred group
	TTGCCTTGTTTATGAGCCATTA
APH11	GGACCTCAGGAAAATCAGTGTA	58.5	183-185	Outbred group
	GCAGGCAGAGCAGGAAATA
APL2	GATTCAACCTTAGCTATCAGTCTCC	58.5	115-125	Outbred group
	CGCTCTTGGCAAATGTCC
CAUD011	TGCTATCCACCCAATAAGTG	50.3	145-223	Outbred group
	CAAAGTTAGCTGGTATCTGC
CAUD006	ATGGTTCTCTGTAGGCAATC	63.5	183-290	Outbred group
	TTCTGCTTGGGCTCTTGGA			Haplotype
CAUD018	TTAGACAAATGAGGAAATAGTA	50.3	100-180	Outbred group
	GTCCAAACTAAATGCAGGC			Haplotype
CAUD010	GGATGTGTTTTTCATTATTGAT	50.3	138-200	Outbred group
	AGAGGCATAAATACTCAGTG			Haplotype
CAUD012	ATTGCCTTTCAGTGGAGTTTC	63.5	182-286	Outbred group
	CGGCTCTAAACACATGAATG			Haplotype
CAUD014	CACAACTGACGGCACAAAGT	58.1	136-200	Outbred group
	CTGAGTTTTTCCCGCCTCTA			Haplotype
CAUD034	TACTGCATATCACTAGAGGA	55.5	160-296	Outbred group
	TAGGCATACTCGGGTTTAG			Haplotype
CAUD035	GTGCCTAACCCTGATGGATG	63.5	174-282	Outbred group
	CTTATCAGATGGGGCTCGGA			Haplotype
APL579	ATTAGAGCAGGAGTTAGGAGAC	55.0	116-227	Outbred group
	GCAAGAAGTGGCTTTTTTC			Haplotype
AY258	ATGTCTGAGTCCTCGGAGC	58.1	89-162	Outbred group
	ACAATAGATTCCAGATGCTGAA			Haplotype
CMO212	CTCCACTAGAACACAGACATT	58.0	186-272	Outbred group
	CATCTTTGGCATTTTGAAG			Haplotype
CAUD028	TACACCCAAGTTTATTCTGAG	55.5	152-226	Outbred group
	ACTCTCCAGGGCACTAGG			Haplotype
CAUD026	ACGTCACATCACCCCACAG	60.8	134-196	Outbred group
	CTTTGCCTCTGGTGAGGTTC			Haplotype
APH18	TTCTGGCCTGATAGGTATGAG	58.0	178-325	Haplotype
	GAATTGGGTGGTTCATACTGT
CAUD002	CTTCGGTGCCTGTCTTAGC	60.8	200-231	Haplotype
	AGCTGCCTGGAGAAGGTCT
CAUD005	CTGGGTTTGGTGGAGCATAA	60.8	184-290	Haplotype
	TACTGGCTGCTTCATTGCTG