Role of Aminoglycoside-Modifying Enzymes (AMEs) in Resistance to Aminoglycosides among Clinical Isolates of Pseudomonas aeruginosa in the North of Iran

Table 1

Primers used for detection of aminoglycoside-modifying enzyme-encoding genes by PCR.

Target genes	Primer sequences (5 to 3)	Amplicon size (bp)	Annealing temperature (°C)	References
ANT(3)-Ia	Pri F: TGT AGA AGT CAC CAT TGT TG	152	51	This study
	Pri R: TCA GCA AGA TAG CCA GAT
ANT(2)-Ia	Pri F: GCA GGT CAC ATT GAT ACA C	225	54	This study
	Pri R: TCC GCT AAG AAT CCA TAG TC
AAC(6)-Ib	Pri F: GAC CAA CAG CAA CGA TTC	375	57	This study
	Pri R: AAC AGC AAC TCA ACC AGA
AAC(6)-IIa	Pri F: CCA TAA CTC TTC GCC TCA T	442	48	This study
	Pri R: AAT CCT GCC TTC TCA TAG C
APH(3)-IIb	Pri F: TTC GTC AAG CAG GAA GTC	662	50	This study
	Pri R: TAG AAG AAC TCG TCC AAT AGC
APH(3)-Ib	Pri F: TTG TTG TTG ACG CAT TGG	284	56	This study
	Pri R: GCC GAC TAC CTT ACC TTC
APH(3)-Ib	Pri F: GGT GAT AAC GGC AAT TCC	548	56	This study
	Pri R: GGT CCA ATC GCA GAT AGA