Downregulation of CFTR Is Involved in the Formation of Hypertrophic Scars

Figure 2

CFTR deficiency can aggravate the formation of hypertrophic scars. (a) Under mechanical loading, wound healing was significantly delayed, and larger hypertrophic scars were detected in ΔF508 mice compared to those in WT mice on day 28. Scar areas were lined out. (b) Masson staining showed that collagen deposition was significantly greater in ΔF508 HTS compared to that in WT HTS. (c) Picrosirius red staining revealed that the ratio of Col1/Col3 was significantly higher in ΔF508 HTS compared to WT HTS. Scale bar = 50 μm. (d): IF staining showed a significantly higher ratio of Col1/Col3 in the HTS of ΔF508 HTS than that of WT HTS. The right panels are the magnification of the left panels. Scale bar = 50 μm. (e) RT-PCR showed that the mRNA expression of TNF-α, IL-1β, IL-6, CCL-2 was significantly higher in ΔF508 mice during scar formation, whereas the mRNA expression of PCNA was similar. TIMP-1 was significantly higher in ΔF508 mice than that in WT mice, especially in the remodeling phase. ; .

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