LPS-Treated Podocytes Polarize Naive CD4⁺ T Cells into Th17 and Treg Cells

Figure 4

LPS-treated podocytes induce naive CD4⁺ T cells to Treg cells (CD4⁺CD25⁺Foxp3⁺ cells). After 48 hours of coculture, suspended cells in each group were collected for further study. (a–d) The levels of Treg cells in the naive CD4⁺ T group (a), BMDC+naive CD4⁺ T cell group (b), PCL cell+naive CD4⁺ T cell group (c), and PCL cell+BMDC+naive CD4⁺ T cell group (d) were calculated by flow cytometry, respectively. (e) Comparison of the levels of Treg cells in total CD4⁺ T cells among groups. (f) Comparison of the Th17/Treg ratio among culture groups. Data are shown as the . . . . /group. BMDC: bone marrow-derived dendritic cell; PCL: podocyte cell line.

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