LPS-Treated Podocytes Polarize Naive CD4⁺ T Cells into Th17 and Treg Cells

Figure 3

LPS-treated podocytes could induce naive CD4⁺ T cells to Th17 cells (CD4⁺IL-17A⁺ cells). LPS-treated PCL cells or BMDCs were cocultured with naive CD4⁺ T cells (obtained by magnetic cell sorting) at a ratio of 1 : 1 or 1 : 1 : 1. One control group (naive CD4⁺ T cells only) and three coculture groups (BMDCs+naive CD4⁺ T cells, PCL cells+naive CD4⁺ T cells, and PCL cells+BMDCs+naive CD4⁺ T cells) were established. After 48 hours, suspended cells in each group were collected for further study. (a–d) The levels of Th17 cells in the naive CD4⁺ T cell group (a), BMDC+naive CD4⁺ T cell group (b), PCL cell+naive CD4⁺ T cell group (c), and PCL cell+BMDC+naive CD4⁺ T cell group (d) were calculated by flow cytometry, respectively. (e) Comparison of the levels of Th17 cells in total CD4⁺ T cells among groups. Data are shown as the . . . /group. BMDC: bone marrow-derived dendritic cell; PCL: podocyte cell line.

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