LPS-Treated Podocytes Polarize Naive CD4⁺ T Cells into Th17 and Treg Cells

Figure 2

The expression of CD80, MHC II, and TLR4 mRNA in LPS-treated BMDCs. BMDCs obtained from C57BL/6 mice were cultured for 6 hours with or without 25 μg/ml LPS. CD80, MHC II, and TLR4 mRNA were examined and compared among the 0 h untreated, 6 h untreated, and 6 h LPS-treated groups. CD80 and MHC II were measured by flow cytometry while TLR4 mRNA by qRT-PCR. (a–c) The levels of CD80 and MHC II expressed in BMDCs in the 0 h untreated group (a), 6 h untreated group (b), and 6 h LPS-treated group (c) were calculated, respectively. (d–g) Comparison of CD80, MHC II, and TLR4 mRNA expressions in BMDCs among groups. Data are shown as the . . /group. BMDCs: bone marrow-derived dendritic cells.

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