Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2

Figure 4

Irisin ameliorates renal I/R damage through regulating UCP2 expression, and UCP2 facilitates I/R-induced renal damage. (a) The mRNA expression of UCP2 in a mouse model subjected to an intraperitoneal injection with different doses of irisin. (b) Western blot illustrating the expression of UCP2 in mice and quantifications of UCP2/β-actin. (c) The mRNA expression of UCP2 in a mouse model fed 1% CLA for various durations. Renal function was evaluated by measuring the (d) SCr and (e) BUN levels. (f, g) Representative H&E images and quantitative analysis of kidney sample tubular injury scores following staining with H&E. Magnification, 400x. (-6 mice in each group). Statistics are presented as . ; . Abbreviations: I/R: ischemia/reperfusion; CTL: control, with no addition of irisin; H/R: hypoxia/reoxygenation; UCP2: uncoupling protein 2; CLA: conjugated linoleic acid; NS: no significant difference.

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