MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB

Figure 5

miR-384 inhibited the progression of PTC cells by targeting PRKACB. (a–c) PRKACB overexpression in K1 cells by real-time PCR analysis and western blot. (d–f) The proliferative ability of the indicated cells detected by MTT assays and soft agar assays. Only cell colonies containing more than 50 cells were counted. Error bars represent mean ± SD from 3 independent experiments. (g, h) Representative images of wound-healing assay (original magnification, ×100). Histograms represent the average migrated distances at the indicated times. Error bars represent mean ± SD from three independent experiments. (i, j) K1/miR-384, K1/miR-384 + PRKACB, and K1/Vector cells were injected into the hind limbs of nude mice (n = 4). Tumor volumes were measured on the indicated days. The tumor volume data were presented as the mean ± SD. (k, l) Histopathological analyses of xenograft tumors. The tumor sections were stained with HE or subjected to IHC staining using an antibody against Ki-67. Error bars represent mean ± SD from three independent experiments. .