MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB

Figure 4

miR-384 decreased PRKACB expression by directly binding to its 3′-UTR. (a) Predicted miR-384 target sequences in the 3′-UTRs of PRKACB and their mutants containing altered nucleotides in the 3′-UTRs. (b–c) Real-time PCR analysis of PRKACB. (d) Western blot analysis of PRKACB as well as the activation of PKA downstream effectors (in the indicated cells). (e) Luciferase assay analyses of the indicated cells transfected with the indicated reporters with increasing amounts of miR-384 (Error bars represent mean ± SD from three independent experiments; ).

(a)

(b)

(c)

(d)

(e)