MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB

Figure 3

Inhibition of endogenous miR-384 promoted the progression of PTC cells. (a) Expression of miR-384 in BCPAP and K1 cells transfected with inhibitor or their paired negative control lentiviral vector by real-time PCR. (b–d) The proliferative ability of the indicated cells detected by MTT assays and soft agar assays. Only cell colonies containing more than 50 cells were counted. Error bars represent mean ± SD from 3 independent experiments. (e–f) Representative images of wound-healing assay (original magnification, ×100). Histograms represent the average migrated distances at the indicated times. Error bars represent mean ± SD from three independent experiments. (g) Transwell migration assay. Representative images (left) and quantification (right) of migrated cells across a Transwell chamber. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)