Tumor-Suppressive Function of lncRNA-MEG3 in Glioma Cells by Regulating miR-6088/SMARCB1 Axis

Figure 3

SMARCB1 on cell proliferation and migration of glioma cells. Note: after U87 and U251 cells were transfected with pcDNA3.1, pcDNA3.1-SMARCB1, si-NC, or si-SMARCB1 plasmid, the mRNA and protein expressions of SMARCB1 were inspected by RT-PCR (a) and Western blot (b), respectively. MTT assay was used to estimate the cell proliferation ability of U87 (c) or U251 (d) cells. The number of clones of U87 or U251 cells was determined by colony formation assay (e-f). Cell migration ability of U87 or U251 cells was tested by cell scratch test (g-h). The mRNA and protein expressions of EMT (E-cadherin, N-cadherin, Vimentin, and Snail-1) in U87 or U251 cells were measured by RT-PCR (i-j) and Western blot (k-l), respectively. , ; EMT, epithelial-mesenchymal transition.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)