Tumor-Suppressive Function of lncRNA-MEG3 in Glioma Cells by Regulating miR-6088/SMARCB1 Axis

Figure 2

MEG3 on cell proliferation and migration of glioma cells. Note: after U87 and U251 cells were transfected with pcDNA3.1, pcDNA3.1-MEG3, si-NC, or si-MEG3 plasmid, mRNA of MEG3 was detected by RT-PCR (a). Proliferation ability of U87 or U251 cells was measured by MTT assay (b-c). The number of clones of U87 or U251 cells was determined by colony formation assay (d-e). Cell migration ability of U87 or U251 cells was tested by cell scratch test (f-g). The mRNA and protein expressions of EMT (E-cadherin, N-cadherin, Vimentin, and Snail-1) in U87 or U251 cells were measured by RT-PCR (h-i) and Western blot (j-k), respectively. , ; MEG3, maternally expressed gene 3; EMT, epithelial-mesenchymal transition.

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