Negative Regulation of Tec Kinase Alleviates LPS-Induced Acute Kidney Injury in Mice via theTLR4/NF-κB Signaling Pathway

Figure 2

LPS-induced Tec kinase expression in kidney tissue and NRK-52E cell. (a, b) The expression of Tec kinase was detected by Western blot in LPS- (20 mg/kg) treated mice at 1, 6, 24, and 48 h. Quantitative analysis of Tec protein was determined by integral optical density (, , ). (c) Immunohistochemical analysis of Tec kinase expression in kidney tissue. . (d) The expression of Tec kinase was detected by Western blot in NRK-52E cell exposed with various concentrations of LPS (0.01, 0.1, 1, and 10 μg/mL). The relative expression was quantified (, ). (e) The expression of Tec kinase was detected in NRK-52E cell after 0.1 μg/mL LPS stimulus at different times. The relative level of Tec kinase was quantified. 0.1 μg/mL LPS increased the Tec expression in a time-dependent manner, with a peak at 60 min (, ).

(a)

(b)

(c)

(d)

(e)