Negative Regulation of Tec Kinase Alleviates LPS-Induced Acute Kidney Injury in Mice via theTLR4/NF-κB Signaling Pathway

Figure 1

LPS-induced acute kidney injury. C57BL/6 mice (male, 8–12 weeks old) were divided into 5 groups and subjected to the following treatment: (1) control (injected with saline); (2) LPS treatment for 1 h; (3) LPS treatment for 6 h; (4) LPS treatment for 24 h; (5) LPS treatment for 48 h. (a–c) Blood samples were collected for BUN, creatinine, and Cys-C measurement. (d) Representative images of HE staining of kidney tissues from control and LPS- (20 mg/kg) treated mice at 1, 6, 12, 24, and 48 h. . (e) Pathological score of representative renal tissue samples of each group. The scoring based on the area of interstitial inflammation and fibrosis, tubular atrophy, and dilation with cast formation involving percentage of the field. 0: normal; 1: <25%; 2: between 25% and 50%; 3: >50%. The score was calculated by averaging the grades assigned to all tubule fields. The data are expressed as the of 5 mice in each group. and , significant difference vs. control.

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