miR-146b-5p Plays a Critical Role in the Regulation of Autophagy in ∆per Brucella melitensis-Infected RAW264.7 Cells

Figure 4

mmu-miR-146b-5p targets Tbc1d14. (a) Tbc1d14 mRNA levels in cells transfected with miR-NC mimic and miR-146b mimic. RAW264.7 were transfected with miR-146b mimic (100 nM), and the Tbc1d14 mRNA levels were measured at 24 h after transfection by qRT-PCR assays. Control (con) cells were transfected with miR-NC mimic. (b, c) Mutation of Tbc1d14 3′UTR. The binding sites of miR-146b in the Tbc1d14 3′UTR (AGUUCUCC) were predicted by bioinformatics software. AGUUCUC (the binding sites) were mutated into CUGGAGU (red). (d) The miR-146b-5p binding sites in mouse Tbc1d14-3′UTR mediates the repression of luciferase activities in cells. Data are mean ± SD from three independent experiments. ; .

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