Isorhamnetin Induces Melanoma Cell Apoptosis via the PI3K/Akt and NF-κB Pathways

Figure 5

Effect of isorhamnetin on NF-κB in B16F10 cells. Cytosolic extracts and nuclear extracts were prepared after incubated with 100 μmol/L IH for 24 h. (a, b) The translocation of NF-κB p65 was detected by Western blotting. All proteins were determined with specific antibodies. The suppressive effect of IH was confirmed by the upregulation of cytosolic NF-κB and downregulation of nuclear NF-κB. The anti-β-actin antibody and anti-Histone H3 were used to check the proper protein loading. Each experiment was repeated at least three times. compared with control.

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