Isorhamnetin Induces Melanoma Cell Apoptosis via the PI3K/Akt and NF-κB Pathways

Figure 4

Effect of isorhamnetin on the PI3K/Akt and NF-κB pathways in B16F10 cells. Cells were pretreated with 100 μmol/L IH for 24 h; then, the cell extracts were blotted with specific antibodies. The phosphorylated protein level of p-Akt was detected. The level of p-Akt and total PI3K was downregulated after IH treatment, and the total level of Akt and NF-κB showed no difference. The anti-β-actin antibody was used to check the proper protein loading. Each experiment was repeated at least three times. compared with control.