The Coumarin Derivative 5′-Hydroxy Auraptene Suppresses Osteoclast Differentiation via Inhibiting MAPK and c-Fos/NFATc1 Pathways

Figure 2

Inhibitory effect of 5′-HA on RANKL-induced osteoclastogenesis in BM cells. (a) Dose-dependent inhibitory effect of 5′-HA on osteoclast differentiation of BM cells as measured by quantification of the total number of TRACP⁺MNCs and (b) quantitative TRAP activity. BM cells were induced to differentiate into osteoclasts without (Ctrl) or with RANKL and M-CSF in the absence (0) or the presence of different concentrations of 5′-HA for 7 days. Images of TRAP staining for MNCs were shown. (c) Dose-dependent inhibitory effect of 5′-HA on osteoclast bone resorption. BM cells were induced with RANKL in the absence or the presence of 5′-HA on fluoresceinated calcium phosphate-coated 24-well plates for 6 days as described in Section 2. Images of pit resorption assay were shown. Values are mean ± SD of three independent experiments (, compared to RANKL-induced cells without 5′-HA).

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