The Coumarin Derivative 5′-Hydroxy Auraptene Suppresses Osteoclast Differentiation via Inhibiting MAPK and c-Fos/NFATc1 Pathways

Figure 1

Cytotoxicity of 5′-HA on RANKL-induced osteoclastogenesis of BM cells. (a) Osteoclast differentiation of mouse BM cells. BM cells (mononuclear cells/macrophages) were induced to differentiate into osteoclasts with RANKL and M-CSF as described in Section 2. The TRACP⁺ cells with more than three nuclei per cell were counted as multinucleated cells (MNCs) at different time points. Images of TRAP staining for MNCs were shown. (b) Quantitative TRAP activity at different time points during osteoclast differentiation of BM cells. (c) Cytotoxicity of 5′-HA on RANKL-induced BM cells. Cells were induced with RANKL and M-CSF in the absence (0) or the presence of different concentrations of 5′-HA and cell viability was measured by CellTiter-Blue® cell viability assay after 3 days in culture. Values are mean ± SD of three independent experiments (, compared to day 1 for (a) and (b) and compared to RANKL-induced cells without 5′-HA for (c)).

(a)

(b)

(c)

(d)