Research Article
Soluble Fiber and Insoluble Fiber Regulate Colonic Microbiota and Barrier Function in a Piglet Model
Table 3
Primer sequences for RT-PCR amplification.
| | Gene1 | Primer sequence (5′–3′)2 | AT3 (°C) | Size (bp) |
| | MUC1 | F: GTGCCGCTGCCCACAACCTG | 60 | 141 | | R: AGCCGGGTACCCCAGACCCA |
| | MUC2 | F: GGTCATGCTGGAGCTGGACAGT | 60 | 181 | | R: TGCCTCCTCGGGGTCGTCAC |
| | CLDN1 | F: GCCACAGCAAGGTATGGTAAC | 60 | 140 | | R: AGTAGGGCACCTCCCAGAAG |
| | OCLN | F: CTACTCGTCCAACGGGAAAG | 60 | 158 | | R: ACGCCTCCAAGTTACCACTG |
| | ZO-1 | F: CAGCCCCCGTACATGGAGA | 60 | 114 | | R: GCGCAGACGGTGTTCATAGTT |
| | IL-10 | F: TAATGCCGAAGGCAGAGAGT | 57.9 | 134 | | R: GGCCTTGCTCTTGTTTTCAC |
| | TNF-α | F: CGTGAAGCTGAAAGACAACCAG | 57.9 | 121 | | R: GATGGTGTGAGTGAGGAAAACG |
| | IL-1β | F: CAGCTGCAAATCTCTCACCA | 53 | 112 | | R: TCTTCATCGGCTTCTCCACT |
| | β-actin | F: TCTGGCACCACACCTTCT | 60 | 114 | | R: TGATCTGGGTCATCTTCTCAC |
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1MUC1: mucin 1; MUC2: mucin 2; CLDN-1: claudin 1; OCLN: occludin; ZO-1: zonula occludens 1; TNF-α: tumor necrosis factor-α. 2F: forward primer; R: reverse primer. 3AT: annealing temperature.
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