Research Article
Soluble Fiber and Insoluble Fiber Regulate Colonic Microbiota and Barrier Function in a Piglet Model
Table 2
Sequences of primers and probes for intestinal bacteria.
| | Bacteria | Primer sequence (5′–3′)1 | AT2 (°C) | Size (bp) |
| | Firmicutes | F: TGAAACTCAAAGGAATTGACG | 60 | 157 | | R: ACCATGCACCACCTGTC |
| | Bacteroidetes | F: GGAACATGTGGTTTAATTCGATGAT | 60 | 126 | | R: AGCTGACGACAACCATGCAG |
| | Roseburia | F: TACTGCATTGGAAACTGTCG | 60 | 230 | | R: CGGCACCGAAGAGCAAT |
| | Prevotella | F: CACCAAGGCGACGATCA | 60 | 283 | | R: GGATAACGCCTGGACCT |
| | Ruminococcus | F: GAGTGAAGTAGAGGTAAGCGGAATTC | 60 | 220 | | R: GCCGTACTCCCCAGGTGG |
| | Universal | F: ACTCCTACGGGAGGCAGCAGT | 60 | 177–179 | | R: GTATTACCGCGGCTGCTGGCAC |
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1F: forward primer; R: reverse primer. 2AT: annealing temperature.
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