Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH against Helicobacter pylori

Figure 3

The H. pylori biofilm treated with OxOral®. The H. pylori biofilm formation was carried using sterile glass coverslips that were placed in Petri dishes. Each plate was filled with MH broth supplemented and OxOral® at different doses: (a) saline solution, (b) 0.938 ppm OxOral®, and (c) 0.469 ppm OxOral®. Biofilm formation was started by inoculating H. pylori at an initial concentration of 1 × 10⁶ CFU/mL. The dishes were incubated under microaerobic conditions at 37°C for 168 h. After incubation, coverslips were washed and stained with 0.1% violet crystal. The coverslips were observed by optical microscopy at a magnification of 100x.

(a)

(b)

(c)